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Registration of Individual Neuron Physiology to Whole Brain Anatomy
The relationship between in vivo measures of neuronal activity and histological structures allows for new insights into the most basic principles of the brain and will have direct applications to disease models. However, due to technology limitations, this relationship has not been established. In this project we propose to develop technology that enables the indentification of functionally assayed individual neurons in cleared whole brains by combining several cutting-edge techniques: endoscopic calcium imaging to obtain the in vivo images, CLARITY brain tissue clearing technique to obtain the histological results, and an image registration algorithm that matches the in vivo neurons to the same set of neurons in the cleared brain images. This project is currently under development. It is in collaboration with Dr. Dave Bulkin from the Department of Neurobiology and Behavior. The project is supported by the Cornell Neurotech Mong Fellowship.
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